Notice
Dr Jeyaraj Ponmozhi - Realtime analysis of drug (diffusion, toxicity, would healing, repair, inflammation, aging with different shear stress studies) on skin cells in a microfluidic skin-on-a-chip device.
- document 1 document 2 document 3
- niveau 1 niveau 2 niveau 3
Descriptif
Advantages of microfluidics could outnumber the advantages in reconstructed and excised skin samples in certain cases for the study on permeability, toxicity, irritation, corrosion, disease models (eg. oral cancer, etc.), pharmacology, therapeutic approaches, pharmacokinetics and formulation optimization with skin cells deposited in the microchannel and could mimic the exact in vivo conditions. Rather constructing the skin models with full-thickness skin, just the skin cells of different layers namely stratum corneum, epidermis (HaCaT), dermis (Fb) & epithelium (EC), etc. can be cultured and deposited or adhered on the microfluidic channels with thickness of about less than 10?m. The deposition of the skin cells can be made by passing the cultured assay at different shear stress as the shear stress controls the porosity of the skin layer thickness we plan to develop for each layers. The drug diffusion can be studied at varied concentration and varied shear stress on single skin cell layers developed with our desired thickness and also overlapping skin cell layers just as the same to mimic the human skin. The skin cells viability can be maintained at our required level for the study (disease models)by providing the nutrients at our required flowrate. The shear stress in the microchannel will affect many factors such as cell-cell communications, extracellular gradients, local concentrations of compounds secreted due to drug reaction with skin cells, the drugs penetration into the skin cells. The results obtained for our skin layer thickness for each type can be extrapolated to the actual thickness of the skin layers for epidermis (32-42?m); dermis (949-1350 ?m); hypodermis (847 – 2979 ?m).
It was reported by different research groups that this microfluidic models has better sensitivity, specificity and accuracy. To highlight the use of microfluidics, the research work by a German group has to be looked through for their work of developing native skin with hair follicles.
Thème
Dans la même collection
-
Dr Julien Chlasta - How’s AFM a fantastic tool in cosmetic research?
Atomic force microscopy (AFM) is a tool for nanoscale analysis-based approach allowing to obtain mechanical information about structures
-
Dr Catherine Grillon - New reconstructed epidermis models closer to skin physiology
Among skin models, reconstructed human epidermis are largely used in cosmetic domain either to evaluate compounds activity or for regulatory tests such as toxicity. They represent a good alternative
-
Dr Franciska Erdo - Studying topical drug delivery in Skin-on-a-chip and by Confocal RAMAN spectros…
Studying skin composition and interaction with topical substances is important both in dermatology and cosmetoscience. Several techniques are utilized and are under development for these purposes.
-
Marek Puskar - Evaluation of serum growth factors in wound healing using a full-thickness in vitro …
Following skin injury, damaged tissue undergoes highly coordinated biological events to restore barrier function involving cross-talk between dermal fibroblasts and epidermal keratinocytes as well as
-
Prof. Dominique J. Lunter - Studying the impact of emulsifiers on SC lipids by confocal Raman micr…
Emulsifiers are widely used in face washes, shower gels, body lotions and many more cosmetic and pharmaceutic products. Some of them are suspected to show irritating effects and to harm the skin
-
Dr Maxim Darvin - Two-photon excited fluorescence lifetime imaging for non-invasive visualization …
Mast cells (MCs) and macrophages (ΜΦs) are important multifunctional immune cells found in all tissues of the body. In the skin, resting and activated MC populations and M1- and M2-polarized ΜΦs are
-
Dr Nikolett Kis - Investigation of the effect of chemical permeation enhancer glycols on the skin m…
Dermal drug delivery is an attractive alternative to conventional drug administration due to its advantages.
-
Dr Miklós Gyöngy - Monitoring of skin using an optical-ultrasound imager for potential cosmetic app…
Optical-ultrasound imaging is a cost-effective method of simultaneously imaging the skin surface and the region under the surface.
-
Michal Szczepanczyk - Catalase activity in keratinocytes, stratum corneum, and defatted algae bioma…
Catalase is one of the most important antioxidative enzymes. Its main function is the decomposition of hydrogen peroxide belonging to the group of reactive oxygen species.
-
Dr Mélanie Pedrazzani - Line-field optical coherence tomography (LC-OCT) assisted by artificial int…
Line-field confocal optical coherence tomography (LC-OCT) is an optical technique based on a combination of confocal microscopy and optical coherence tomography, allowing three-dimensional (3D)
-
Lena Waszczuk - Morpho-molecular characterization of tattooed skin biopsies with adverse reactions …
Line-field confocal optical coherence tomography (LC-OCT) is a non-invasive optical technique for imaging the skin at high resolution (∼ 1 μm), based on a combination of OCT and reflectance confocal
-
Dr Mais Saleh - Nanostructured Vitamin E Phosphate: Characterizing its percutaneous Penetration int…
Modern topical sunscreens combine topical antioxidants with physical UV filters to achieve optimal skin protection1–3. α-Tocopherol phosphate (α-TP), a new pro-vitamin E antioxidant, prevents UVA1
