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Campus Croix Rouge - Université de Reims Champagne-Ardenne Langue : Français
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Camille Depenveiller (Intervention)
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Citer cette ressource :
Camille Depenveiller. JCAD. (2023, 3 octobre). Using High Performance Computing to decipher the impact of ageing process on collagens. [Vidéo]. Canal-U. https://www.canal-u.tv/148423. (Consultée le 23 février 2024)

Using High Performance Computing to decipher the impact of ageing process on collagens

Réalisation : 3 octobre 2023 - Mise en ligne : 27 novembre 2023
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Descriptif

Plant cells have to face environmental stress, and in this context, being able to decipher the organization of the plant plasma membrane is a key point to better understand this adaptability of plasma membranes in immune signaling, host-pathogen interactions or plant-microorganism interactions for instance.

Experimental procedures allow to study the composition and some properties of plant plasma membranes. Biophysical characterization of plasma membrane leaflets has already been carried out [1] [2]. Besides, in combination with this experimental data, molecular dynamics simulations are a very interesting way to investigate the dynamical behavior of lipids in membranes. Atomistic simulations are widely used but for increased system sizes, an approach called coarse-grained simulations, consisting of considering several atoms as one bead, is very useful and allows to reach larger scales. By this way, a model of animal plasma membrane has already been published [3]. However, the plasma membrane of plants is very poorly studied in modeling and simulation, which is particularly due to the lack of plant-specific lipids in databases. The use of high-performance computing can help to simulate larger systems in longer time duration.

The aim of our work is to be able to study the behavior of lipids in a plant plasma membrane. After preparing structures and topologies of the required lipids, membrane models of different compositions are simulated, therefore we run numerous molecular dynamics simulations on one node with CPU Intel Skylake and GPU NVIDIA P100. This work involves researchers from several different labs. This collaboration allows to get competences in experimental biophysics as well as modeling in this transdisciplinary project. Together with the use of data generated by experiments, notably lipid composition and biophysical properties of both plasma membrane leaflets, we combine atomistic and coarse-grained approaches to get insights into the characteristics and behavior of a model of plant plasma membrane. Indeed, we are able to get global membrane properties such as area per lipid, lipid tail order or bilayer thickness. Moreover, we investigate the dynamics and interactions of lipids of the two leaflets. Indeed, thanks to high-performance computing, this work allows to provide knowledge about leaflet communication, lipid-lipid interactions and lipid behavior in a plant plasma membrane.

 Funding

This work has been funded by the ANR PLAYMOBIL.

 References

[1] K. Grosjean et al., ‘Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells', Journal of Experimental Botany, vol. 69, no. 15, pp. 3545–3557, Jun. 2018, doi: 10.1093/jxb/ery152.

[2] A. Mamode Cassim et al., ‘Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation', Journal of Biological Chemistry, vol. 296, p. 100602, Jan. 2021, doi: 10.1016/j.jbc.2021.100602.

[3] H. I. Ingólfsson et al., ‘Lipid Organization of the Plasma Membrane', J. Am. Chem. Soc., vol. 136, no. 41, pp. 14554–14559, Oct. 2014, doi: 10.1021/ja507832e.

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