Director Jason Potter - What matters for making highly expressed mRNA.
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The need for high quality mRNA for therapeutic applications has grown substantially over the last few years due to the efficacy of the COVID mRNA vaccines. The expanding mRNA applications generally use custom mRNA made by IVT systems. Here I will discuss what factors matter for generating mRNA that can be expressed at high levels in mammalian cells. This will include how to optimize yields, capping efficiency, and transcript purity including the removal of immunogenic byproducts. By controlling the quality of mRNA transcripts we have been able to increase expression levels in cells and mice by over 10 fold.
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