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Prof. Maxwell Hincke - The chroriallantoic membrane: insight from proteomics.
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Descriptif
In oviparous animals such as birds, embryonicdevelopment occurs in the egg; after oviposition, there is no furtherpossibility of material exchange from the hen to fulfill the physiologicalneeds of the embryo. In such a context, the egg must contain all resourcesrequired for survival and proper development of a living organism. Duringembryonic development, the chorioallantoic membrane (CAM) is a placenta-likestructure which is the nexus for many different physiological and metabolicprocesses including acid-base balance, respiration, and calcium solubilizationfrom the eggshell that is re-allocated for bone and tissue formation. Thehighly vascularized CAM occupies a strategic position, as it forms a liningunder the eggshell and surrounds the embryo from ED12 onwards. The cellular andgenetic bases for its protective mechanisms remain to be fully elucidated. One approach to understanding the functions ofthe CAM is to identify its protein constituents and how they change duringdevelopment. In this study, we have characterized the CAM proteome at twostages of development (ED12 and ED19), and assessed the embryonic blood serumproteome to determine its contribution. LC/MS/MS-based proteomics allowed theidentification of 1470, 1445, and 791 proteins in CAM (ED12), CAM (ED19), andembryonic blood serum (EBS), respectively. In total, 1796 proteins wereidentified in the entire study. Of these, 175 (ED12), 177 (ED19), and 105 (EBS)were specific to these stages / compartments.
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