Dr Paweł Siudem - Aronia melanocarpa Fruits as a Dietary Source of bioactive compounds: 1H‑NMR, HPLC-DAD, and Chemometric Studies

Aronia melanocarpa (Michx.) Elliott's (chokeberry) it is well knownfor the presence of anthocyanins. The fruits also  contain significant amounts of otherbioactive compounds, such as chlorogenic acid and its isomer neochlorogenicacid. They exhibit antioxidant, anti-inflammatory, antidiabetic, andantibacterial activities; thus, they can have a significant impact on thehealth-promoting properties of chokeberry. In our research, we have determinedthe changes in the content of chlorogenic acids (CGA) and anthocyanins duringthe development and ripening of fruits. Aronia fruit samples from two organicfarms in Poland, were collected every two weeks from July to October. Themethods of NMR spectroscopy and HPLC-DAD were used to determine the chemicalcomposition of the prepared methanolic extracts. The obtained results wereanalyzed using chemometric analysis and multivariate statistics (PCA). Theresults showed that the content of chlorogenic acids and anthocyanins changesduring ripening and depends on the time of harvest and the region ofcultivation. A correlation between the time of CGAs reduction and the appearanceof anthocyanins was also noticed. Additionally, the antioxidant properties ofextracts were determined using three methods: DPPH, FRAP and ORAC.