Canal-U

Mon compte
LE STUDIUM Loire Valley Institute for Advanced Studies

Dr Aurélie Crabbé - Host metabolites modulate bacterial susceptibility to antibiotics


Copier le code pour partager la vidéo :
<div style="position:relative;padding-bottom:56.25%;padding-top:10px;height:0;overflow:hidden;"><iframe src="https://www.canal-u.tv/video/le_studium/embed.1/dr_aurelie_crabbe_host_metabolites_modulate_bacterial_susceptibility_to_antibiotics.57541?width=100%&amp;height=100%" style="position:absolute;top:0;left:0;width:100%;height: 100%;" width="550" height="306" frameborder="0" allowfullscreen scrolling="no"></iframe></div> Si vous souhaitez partager une séquence, indiquez le début de celle-ci , et copiez le code : h m s
Contacter le contributeur
J’aime
Imprimer
partager facebook twitter

Dr Aurélie Crabbé - Host metabolites modulate bacterial susceptibility to antibiotics

Antibiotic susceptibility of bacterial pathogens is typically evaluated using in vitro assays that do not consider the complex host microenvironment. This may help explaining a significant discrepancy between antibiotic efficacy in vitro and in vivo for chronic lung infections, with some antibiotics being effective in vitro but not in vivo or vice versa. We previously demonstrated that lung epithelial cells enhance the activity of aminoglycoside antibiotics against the opportunistic pathogen Pseudomonas aeruginosa, yet the mechanism behind was unknown. I will present mechanistic insights on how lung epithelial cells stimulate aminoglycoside activity. To this end, an in vivo-like 3-D lung epithelial cell model was used. We report that conditioned medium of 3-D lung cells, containing secreted but not cellular components, potentiated the bactericidal activity of aminoglycosides against P. aeruginosa, including resistant clinical isolates, and several other pathogens. We found that 3-D lung cells secreted endogenous metabolites that modulate aminoglycoside activity, and provide evidence on the mode of action. Our findings reveal a cross-talk between host and bacterial metabolic pathways, that influence downstream activity of antibiotics. Understanding the underlying basis of the discrepancy between the activity of antibiotics in vitro and in vivo may lead to improved diagnostic approaches and pave the way towards novel means to stimulate antibiotic activity.

 


  •  
  •  
    Date de réalisation : 25 Septembre 2020
    Durée du programme : 30 min
    Classification Dewey : Maladies des poumons (hypertension artérielle pulmonaire, hypertension pulmonaire, maladie obstructive respiratoire chronique, maladies respiratoires, ouvrages généraux sur les maladies des bronches et des poumons)
  •  
    Catégorie : Conférences
    Niveau : Tous publics / hors niveau
    Disciplines : Clinique médicale
    Collections : LE STUDIUM Conference, Novel host- and microbiota-directed strategies for treating respiratory infections - 24 & 25 September 2020
    ficheLom : Voir la fiche LOM
  •  
  •  
    Langue : Anglais
    Mots-clés : pathologie des poumons, antibiotique
 

commentaires


Ajouter un commentaire Lire les commentaires
*Les champs suivis d’un astérisque sont obligatoires.
Aucun commentaire sur cette vidéo pour le moment (les commentaires font l’objet d’une modération)
 

Dans la même collection

FMSH
 
Facebook Twitter
Mon Compte