Conférence
Notice
Langue :
Anglais
Conditions d'utilisation
Droit commun de la propriété intellectuelle
DOI : 10.60527/k8v9-tb61
Citer cette ressource :
LESTUDIUM. (2021, 30 novembre). Prof. Adrian Wolstenholme - C. elegans as a model for ascarid parasites; levamisole receptors , in New approaches to get around roundworms. [Vidéo]. Canal-U. https://doi.org/10.60527/k8v9-tb61. (Consultée le 19 mars 2024)

Prof. Adrian Wolstenholme - C. elegans as a model for ascarid parasites; levamisole receptors

Réalisation : 30 novembre 2021 - Mise en ligne : 6 décembre 2021
  • document 1 document 2 document 3
  • niveau 1 niveau 2 niveau 3
Descriptif

Ligand-gated ion channels provide an excellent test for any expression system and for C. elegans as a model for parasitic nematode species, including the ascarids. They are complex proteins made of multiple subunits which are encoded by a large gene family, that carry multiple post-translational modifications and which exhibit varied physiological and pharmacological properties. This last property makes them excellent drug targets and provides a fairly simple way of testing the accuracy of any comparison between native and recombinant channels. The levamisole receptor is an excellent illustration; in C. elegans full expression of a levamisole-sensitive receptor in Xenopus oocytes is reported to require 8 proteins, 5 subunits and 3 chaperones. Some years ago we were able to generate levamisole-sensitive receptors by expressing just two subunits. We have also tried to rescue C. elegans mutant strains by over-expression of parasite subunits; whereas UNC-38 and UNC-29 from the clade V parasite H. contortus were able to rescue the mutations, those from Ascaris were less effective. Overall, my feeling is that although C. elegans is a convenient experimental system in many ways, its usefulness for ascaris research will vary on a gene by gene basis, which makes routine prediction and extrapolation unreliable.

Dans la même collection

Sur le même thème