Canal-U

3.5. Making the predictions more reliable

We have got a bacterial gene predictor but the way this predictor works is rather crude and if we want to have more reliable results, we have to inject into this algorithmmore biological knowledge. We will use a notion of RBS, RBS stands for Ribosome Binding Sites. What is it? OK. Let's have a look atthe cell machinery or part of it here. You certainly see here that wedeal with a eukaryotes cell. Why? It's because you have anucleus and you remember that the difference between prokaryoticcell and eukaryotic cell lies n the existence of a nucleus. Within the nucleus you have the DNA. The DNA is transcribed into RNA,OK this is well understood, which goes out of the nucleus and there in the cytoplasm it can be read by a large moleculewhich is called the ribosome. It is within the ribosome that the translation of INA into a protein takes place, the ribosome. The ribosome has to be fixed to the DNA to initiate the translation. The place where this fixation takes placeis named the Ribosome Binding Site. It is a part of the RNA sequence,so the idea here will be to say ‘Well the main problem inpredicting the coding region with our algorithm lies in the fact that we tend to always take as a start codon the left most codonso that the predicting coding region is of a maximum length.